• Name
• Description
• Inherits from
• Attributes
• Methods
  • Standard methods
• Evidence Tags

Name

SWISS::DEs.pm

Description

SWISS::DEs represents the DE lines of a UniProt Knowledgebase (Swiss-Prot + TrEMBL) entry as specified in the user manual http://www.expasy.org/sprot/userman.html.

The DEs object basically holds lists of DE objects, each of them representing a protein name element. The elements, hasFragment, Includes and Contains attributes/methods work as follows :

 DE   RecName: Full=CAD protein;
 DE            Short=CAD;
 DE   AltName: Full=Protein rudimentary;
 DE   Includes:
 DE     RecName: Full=Glutamine-dependent carbamoyl-phosphate synthase;
 DE              EC=6.3.5.5;
 DE   Includes:
 DE     RecName: Full=Aspartate carbamoyltransferase;
 DE              EC=2.1.3.2;
 DE   Flags: Fragment;
 
 -= Entry::DEs =-
 elements (for each DE object, see SWISS::DE.pm documentation) :
    toText:    "CAD protein",  "CAD",       "Protein rudimentary"
    category:  "RecName",      "RecName",   "AltName"
    type:      "Full",         "Short"      "Full"    
 hasFragment : "Fragment"
 Includes : ListBase of DEs (child1, child2)
 Contains : empty ListBase
 
 -= child1 =-    
 elements (for each DE object) :
    toText:    "Glutamine-dependent carbamoyl-
                phosphate synthase",            "6.3.5.5"
    category:  "RecName",                       "RecName",
    type:      "Full",                          "EC"   
 hasFragment : undef
 
 -= child2 =-    
 elements (for each DE object) :
    toText:    "Aspartate carbamoyltransferase",  "2.1.3.2"
    category:  "RecName",                         "RecName",
    type:      "Full",                            "EC"  
 hasFragment : undef

Note: the old unstructured DE format can still be used, and will be parsed the same way into DE objects (but without setting their attributes 'category' and 'type'.

 DE   CAD protein (Protein rudimentary) [Includes: Glutamine-dependent
 DE   carbamoyl-phosphate synthase (EC 6.3.5.5); Aspartate
 DE   carbamoyltransferase (EC 2.1.3.2)] (Fragment). 

Inherits from

SWISS::ListBase.pm

Attributes

text

The (raw) text of the DE line (without the 'DE ' line type prefixes)

list

Array reference to the SWISS::DE objects containing the different names for the entry. The first element of the list is the recommended name. Note: use elements method (inherited from ListBase) to get (and loop through) the array of DE objetcs.

Includes

Contains

Each of these is a SWISS::ListBase object whose list contains a SWISS::DEs object for each 'child' of the protein (i.e. peptide or functional domain). See the UniProtKB user manual for an explanation. It is possible to have both Includes and Contains in a single entry:

 DE   RecName: Full=Arginine biosynthesis bifunctional protein argJ;
 DE   Includes:
 DE     RecName: Full=Glutamate N-acetyltransferase;
 DE              EC=2.3.1.35;
 DE     AltName: Full=Ornithine acetyltransferase;
 DE              Short=OATase;
 DE     AltName: Full=Ornithine transacetylase;
 DE   Includes:
 DE     RecName: Full=Amino-acid acetyltransferase;
 DE              EC=2.3.1.1;
 DE     AltName: Full=N-acetylglutamate synthase;
 DE              Short=AGS;
 DE     RecName: Full=Arginine biosynthesis bifunctional protein argJ alpha chain;
 DE   Contains:
 DE     RecName: Full=Arginine biosynthesis bifunctional protein argJ beta chain;

hasFragment

Contains 'Fragment' or 'Fragments' (evaluates to true) if the DE lines contain the 'Fragment(s)' indication (in 'Flags:' line with the new DE line format), otherwise evaluates to false. Compare to the more robust Entry::isFragment which also checks the FT lines for a NON_CONS or NON_TER.

isPrecursor

Returns 1 if the flag 'Precursor' is present (undef if not). Note: only with new DE line format.

Methods

Standard methods

new

fromText

toText

Evidence Tags

With the new DE line format, each DE element can have distinct evidence tags, which are stored in the DE object themself (see SWISS::DE.pm documentation). The evidence tags for the 'Flags' line are stored in the parent DEs object itself. With the old DE line format, since the DE line did not have a fixed syntax in TrEMBL, it is impossible to reliably assign evidence tags separately to the different elements of the DE lines. Therefore, the DE line can only be evidence tagged as a whole, and the following methods have their prototype defined in SWISS::BaseClass instead of the direct parent of SWISS::DEs, SWISS::ListBase :

 addEvidenceTag
 deleteEvidenceTags
 getEvidenceTags
 getEvidenceTagsString
 hasEvidenceTag
 setEvidenceTags

example :

 $evidenceTag = $entry->Stars->EV->addEvidence('P', 'DEfix', '-', 'v1.3');
 # add global DE evtag if old DE line format, 'Flags' evtag if new format
 $entry -> DEs -> addEvidenceTag($evidenceTag);